High expression vectors for a Zygosaccharomyces rouxii host.

ZrFsy1, a High-Affinity Fructose/H+ Symporter from Fructophilic Yeast Zygosaccharomyces rouxii

Zygosaccharomyces rouxii is a fructophilic yeast than can grow at very high sugar concentrations. We have identified an ORF encoding a putative fructose/H(+) symporter in the Z. rouxii CBS 732 genome database. Heterologous expression of this ORF in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic characterization of its sugar transport activity showed...

Recent allopolyploid origin of Zygosaccharomyces rouxii strain ATCC 42981.

Zygosaccharomyces rouxii strain ATCC 42981 has been reported to have two copies of several genes including HOG1 and SOD2, whereas the type strain of Z. rouxii (CBS 732) has only one. To investigate the structure of the ATCC 42981 genome we sequenced random fragments from this genome and compared the data to the type strain. We found that ATCC 42981 contains two versions of the ribosomal RNA arr...

Evaluation of different chemical preservatives to control Zygosaccharomyces rouxii growth in high sugar culture media

Construction of High Ethanol Fermenting Halotolerant Hybrid by Intergeneric Protoplast Fusion of Saccharomyces cerevisiae and Zygosaccharomyces rouxii

Saccharomyces cerevisiae M30, a high ethanol producing flocculent strain, was hybridized with Zygosaccharomyces rouxii TISTR1750, a halotolerant non-flocculent strain, by polyethylene glycol induced protoplast fusion. The protoplast fusion of these two yeast genera occurred with frequency of 5.89X10-5. Three fusants, namely RM11, RM37 and RM58, were selected based on their high ethanol fermenta...

Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells.

Recombinase encoded by the R gene of pSR1 of Zygosaccharomyces rouxii mediates reciprocal recombination between two specific recombination sites (RSs) to induce excision or inversion of the DNA segment that is flanked by the RSs. We report here that site-specific recombination mediated by this system takes place effeciently in tobacco cells. To monitor the recombination events in tobacco cells,...